The application provides a mechanism to create a dark frames and bad pixel maps for the connected camera by taking and evaluating images of the covered camera. The dark frame / bad pixel map can then be used to remove / repair hot and cold pixel of acquired images. In general it is not recommended to use both dark frame subtraction as well as a bad pixel map at the same time.
Instructions on how to create a dark frame / bad pixel map file can be found here: Bad Pixel Map / Dark Frame Creation